Journal: JAC-Antimicrobial Resistance

Article Title: Genetic and clinical landscape of methicillin-resistant Staphylococcus aureus isolates lacking the mec gene, in Japan

doi: 10.1093/jacamr/dlag008

Figure Lengend Snippet: A single-nucleotide polymorphism-based phylogenetic tree of methicillin resistance-lacking mec (MRLM) strains of Staphylococcus aureus . The whole genome sequences of all 13 strains analysed in this study (in red) were examined alongside those of 150 published MRLM strains, , downloaded from the NCBI database. The following MSSA genome sequences were also included: seven reference strains ATCC 25923 (ST243/CC30, CP009361.1), ATCC 29213 (ST5, GCA_001267715.2), ATCC 12600 (ST8, GCA_006094915.1), ATCC 9144 (ST30, GCA_900457695.1), NCTC 8325 (ST8, GCA_000013425.1), MSSA476 (ST1, GCA_000011525.1) and MSSA6850 (ST50, GCA_000462955.1), and eight previously reported clinical MSSA strains 10-00598 (ST22, GCA_028076695.1), 15-02736 (ST291, GCA_028082935.1), 16-01080 (ST45, GCA_028080235.1), 16-02665 (ST25, GCA_028075835.1), 18-01695 (ST398, GCA_028081975.1), 18-01787 (ST5, GCA_028081295.1), 19-00515 (ST15, GCA_028081935.1), and C00012754 (ST20, ERR418969). , Sequence types (STs) (inner ring), clonal complexes (CCs) (middle ring) and spa types (outer ring) are annotated. The tree scale indicates the number of substitutions per genome.

Article Snippet: A comparative analysis of the nucleotide sequences or deduced amino acid sequences extracted from 13 MRLMs with those from the MSSA reference strain ATCC 25923 (ST243/CC30, CP009361.1) was conducted to identify mutations in the pbp4 promoter and amino acid substitutions in PBPs 1-4, GdpP, YjbH and AcrB.

Techniques: Sequencing

Journal: bioRxiv

Article Title: BAY 11-7082 potentiates select β-lactams to inhibit growth of methicillin-resistant Staphylococcus aureus

doi: 10.64898/2026.01.13.699313

Figure Lengend Snippet: A. MRSA expresses five PBPs, PBP1, PBP2, PBP2A (pink), PBP3, and PBP4. B. FICI values calculated based on MRSA USA300 grown in the presence of BAY 11-7082 and another antibiotic. FICI values <0.5 represent synergy, values between 0.5-4 represent no interaction, and values >4 represent antagonism. C. FICI values for MRSA USA300 (pink) and MSSA ATCC 29213 (blue) grown in the presence of BAY 11-7082 and a β-lactam that synergizes with BAY 11-7082 in MRSA. Data represent an average of three independent experiments performed in triplicate.

Article Snippet: We conducted checkerboard assays with MRSA strain USA300, which has mecA ( , Figure S2 ), as well as methicillin-sensitive S. aureus (MSSA) strain ATCC 29213, which does not, to understand the potential role of PBP2a ( , Figure S3 ).

Techniques:

Journal: ACS Omega

Article Title: Z‑Amino Acids Present Innovative Antimicrobial and Antibiofilm Properties against Methicillin-Susceptible and -Resistant Staphylococcus aureus

doi: 10.1021/acsomega.5c09099

Figure Lengend Snippet: Log 10 CFU of planktonic S. aureus following treatment with Z- and L-amino acids. (A) MSSA and (B) MRSA treated with Z-amino acids at various concentrations. (C) MSSA and MRSA treated with Z-Gln, Z-Gly, and Z-Ser at 0.5× MIC. (D) MSSA and (E) MRSA treated with Z-Gln, l -Gln, Z-Gly, and l -Gly at 0.5× MIC. Statistical significance: ** < 0.01 and **** < 0.0001 compared to the other Z-amino acids (A, B), MSSA vs MRSA (C), and their corresponding L-amino acids (D, E); c < 0.001 and d < 0.0001 compared to the control. Data are represented as the mean ± standard deviation with n = 3 replicates.

Article Snippet: MSSA (ATCC 25923) and MRSA (BAA 1717) were purchased from ATCC (Manassas, VA) and studied.

Techniques: Control, Standard Deviation

Journal: ACS Omega

Article Title: Z‑Amino Acids Present Innovative Antimicrobial and Antibiofilm Properties against Methicillin-Susceptible and -Resistant Staphylococcus aureus

doi: 10.1021/acsomega.5c09099

Figure Lengend Snippet: Bacterial inhibition kinetics of S. aureus after treatment with Z-amino acids at 1× MIC. Percent inhibition of (A) MSSA and (B) MRSA. Data are represented as the mean ± standard deviation with n = 3 replicates.

Article Snippet: MSSA (ATCC 25923) and MRSA (BAA 1717) were purchased from ATCC (Manassas, VA) and studied.

Techniques: Inhibition, Standard Deviation

Journal: ACS Omega

Article Title: Z‑Amino Acids Present Innovative Antimicrobial and Antibiofilm Properties against Methicillin-Susceptible and -Resistant Staphylococcus aureus

doi: 10.1021/acsomega.5c09099

Figure Lengend Snippet: Relative percent of biofilm biomass of (A) MSSA and (B) MRSA treated with Z-amino acids at various concentrations. Comparison in relative percent of biofilm biomass (C) between MSSA and MRSA treated with Z-amino acids at 0.5× MIC and (D) between the treatment of Z-amino acids and L-amino acids against MSSA at 1× MIC. Statistical significance: * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001 compared to the other Z-amino acids (A–C) or their corresponding L-amino acids (D); b < 0.01, c < 0.001, and d < 0.0001 compared to the control (A, B, D). Data are represented as the mean ± standard deviation with n = 6 replicates.

Article Snippet: MSSA (ATCC 25923) and MRSA (BAA 1717) were purchased from ATCC (Manassas, VA) and studied.

Techniques: Comparison, Control, Standard Deviation

Journal: ACS Omega

Article Title: Z‑Amino Acids Present Innovative Antimicrobial and Antibiofilm Properties against Methicillin-Susceptible and -Resistant Staphylococcus aureus

doi: 10.1021/acsomega.5c09099

Figure Lengend Snippet: Relative percent of biofilm biomass of (A) MSSA biofilm dispersal after treatment with Z-Gln, Z-Gly, and Z-Ser and (B) representative images of wells stained with crystal violet. (C) MRSA biofilm dispersal after treatment with Z-Gln, Z-Gly, and Z-Ser. (D) Representative images of wells stained with crystal violet. Statistical significance: *** < 0.001, **** < 0.0001; b < 0.01, c < 0.001, and d < 0.0001 compared to the control. Data are represented as the mean ± standard deviation with n = 6 replicates.

Article Snippet: MSSA (ATCC 25923) and MRSA (BAA 1717) were purchased from ATCC (Manassas, VA) and studied.

Techniques: Staining, Control, Standard Deviation